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Proteomics

One-dimensional electrophoresis

– Amersham Biosciences gel SE 600 Ruby vertical plate gel electrophoresis unit

– GE Healthcare EPS 601 Electrophoresis Power Supply, for electrophoresis applications requiring a maximum of 600V, 100W and 400mA.

Applications: separation of proteins and fractionation of nucleic acids under denaturing and non-denaturing conditions.

Two-dimensional electrophoresis

The system comprises the Ettan IPGphor unit combined with independent strip holders. IEF separation protocols can be programmed, run and controlled from the Ettan IPGphor control panel or from a PC running the software.

Applications: realization of the first dimension in the case of 2-D electrophoresis. It allows fractionation based on the isoelectric point of proteins (isoelectric focusing or isoelectric focusing, IEF).

Eppendorf BioSpectrometer® basic UV/Vis spectrophotometer

Specially designed for using in the fields of molecular biology, biotechnology, biochemistry and cell biology. It allows measurement in a wavelength range from 200 nm to 830 nm and the use of glass and plastic cuvettes in a volume range from 1 µL to 3000 µL.